摘要Herpes simplex virus type 1(HSV-1)infects over 70％of the population and establishes lifelong latent infection with periodic reactivation in humans,resulting in various related diseases.However,the molecular and cellular events underlying the transition of HSV-1 from latency to reactivation remain poorly understood.In this study,we used bulk RNA sequencing and single-cell transcriptomic ana-lyses to dissect the cellular and molecular events of HSV-1 latency-reactivation transition in infected trigeminal ganglia(TG)in both mouse and tree shrew infection models.We found that mice exhibited fluctuating host gene responses during the acute phase and relatively quiescent latency,whereas tree shrews displayed a relatively mild acute phase and active latency characteristics.Single-cell analysis revealed that HSV-1 infects TG neuronal subpopulations expressing growth hormone and pituitary hormones.Importantly,we observed that HSV-1 latency in tree shrew TGs exhibited inhibition of cellular autophagy function,while HSV-1 latency in mice was accompanied by the attenuation of monocyte-related immune surveillance.Given that infected cell protein 0(ICP0)has autophagy inhibitory activity,we further investigated the role of this viral protein in tree shrew models using an ICP0-deficient HSV-1 strain.Notably,the mutant virus could not undergo spontaneous reactivation from latency.These findings support the hypothesis that ICP0 may be essential for spontaneous reactivation by inhibiting autophagy in vivo.