摘要目的观察血管紧张素-Ⅱ（Ang-Ⅱ）对大鼠胚胎心纤维母细胞（FBC）胶原合成过程中癌基因c-fos mRNA及前胶原基因α1［Ⅰ］(pro-α1［Ⅰ］)mRNA表达的影响。方法用培养大鼠胚胎心FBC，以荧光法检测Ang-Ⅱ引起的FBC内游离钙浓度变化，用RT-PCR法检测c-fos mRNA及pro-α1［Ⅰ］mRNA表达。结果①Ang-Ⅱ 10-8～10-5使FBC内游离钙浓度呈剂量依赖性增加；②PCR显示，FBC经Ang-Ⅱ 10-7～10-6mol/L作用后，c-fos mRNA和pro-α1［Ⅰ］mRNA表达增强。结论 Ang-Ⅱ通过活化FBC内信号系统诱导c-fos mRNA及pro-α1［Ⅰ］mRNA的表达。

abstractsObjective　To observe the effects of Ang-Ⅱ on expressions of c-fos mRNA and pro-α1［Ⅰ］mRNA in fibroblasts (FBC) of rat embryo in the course of collagen synthesis.Methods　The change of free Ca2+(［Ca2+］i) was tested in fluorometry and the expressions of c-fos mRNA and pro-α1［Ⅰ］mRNA were tested by RT-PCR method in incubated FBC.Results　The level of［Ca2+］i in FBC and the expressions of c-fos mRNA and pro-α1［Ⅰ］mRNA were enhanced with the increase of Ang-Ⅱ concentration(10-8～10-5and 10-7～10-6mol/L).Conclusions　The expressions of c-fos mRNA and pro-α1［Ⅰ］mRNA were induced by Ang-Ⅱ activating signal system in FBC.