摘要目的 用受试者工作特征曲线(receiver operating characteristic curve,简称ROC曲线)法评价上转发光技术(UPT)快速检测鼠疫抗原抗体的效果,为UPT快速检测技术的鼠疫现场应用提供依据.方法 在喜马拉雅旱獭和阿拉善黄鼠疫源地采集动物血清标本224份,用UPT、酶联免疫吸附试验(ELISA)、金标、间接血凝试验(IHA)4种方法检测鼠疫菌F1抗体；采集旱獭、黄鼠脏器、骨髓标本108份,用UPT、ELISA、金标、PCR、反向间接血凝试验(RIHA)5种方法检测鼠疫菌F1抗原.抗体检测结果以IHA为标准,抗原检测结果分别以RIHA 、PCR+金标、PCR+ ELISA为标准,结果应用ROC法进行评价.结果 抗体检测UPT、ELISA、金标3种方法的曲线下面积(AUC)均＞0.5,UPT的AUC与其他方法比较,差异无统计学意义(z=1.204,P＞ 0.05).抗原检测UPT、ELISA、金标、PCR 4种方法AUC均＞0.5,UPT的AUC与其他方法比较,差异无统计学意义(z=0.866,P＞ 0.05).结论 UPT快速检测技术对鼠疫抗原抗体检测效果良好,该技术操作简单,快速,结果准确,适合鼠疫现场监测、突发鼠疫疫情应急处理,适宜推广应用.

abstractsObjective To evaluate the effect of up-converting phosphor technology(UPT) in detection of plague antigen-antibody by receiver operating characteristic curve (ROC) method,and to provide a scientific basis for field application of UPT rapid detection technology in plague prevention and control.Methods Two hundred and twenty four serum samples were collected from Marmots and ground squirrels in the plague foci,Yersinia pestis antibody was detected by UPT,ELISA,Colloidal-gold Strips and IHA,respectively; 108 organs and bone marrow samples were collected,and Yersinia pestis antigens were detected by UPT,ELISA,PCR and RIHA,respectively.IHA was used as the gold standard for antibody test results,RIHA,PCR + Colloidal-gold Strips,PCR + ELISA were used as the gold standard for antigen test results.The results were evaluated using ROC method.Results Antibodies detection:the AUCs of UPT,ELISA and Colloidal-gold Strips were greater than 0.5.The difference between UPT and other methods was not statistically significant (z =1.204,P ＞ 0.05).Antigen detection:the AUCs of UPT,ELISA,Colloidal-gold Strips and PCR were greater than 0.5.There was no statistical difference between UPT and other methods(z =0.866,P ＞ 0.05).Conclusions UPT as a new technology works well in the detection of plague antigen-antibody.The technology is simple,fast,accurate,and suitable for on-site monitoring of plague,emergency treatment of sudden plague,and suitable for promotion.