摘要目的 研究青岛市布鲁杆菌病的细菌学鉴定及相关临床特征.方法 回顾性分析青岛大学附属医院2010年4月至2014年11月确诊的34位布鲁杆菌病病原学阳性患者的临床资料,包括患者的一般情况、临床症状、细菌培养及实验室检查等.结果 34例布鲁杆菌病患者年龄为12～71岁,其中男性24例、女性10例;主要临床表现为发热、多汗、关节疼痛常合并多器官症状;26例患者布鲁杆菌血培养阳性、6例组织培养阳性、2例2种培养均阳性,经鉴定34例均为布鲁杆菌属;实验室检查多见肝功能异常、贫血、C反应蛋白和血沉的升高;联合应用抗生素治疗,效果良好.结论 布鲁杆菌病在疾病的非流行区日益增多,应重视细菌学培养等确诊试验,掌握布鲁杆菌的形态及培养特性,避免实验室暴露.

abstractsObjective To investigate the bacteria identification and clinical features of brucellosis in Qingdao City.Methods A retrospective analysis was performed on the clinical data,including general situation,clinical symptom,bacterial culture and laboratory findings,etc of 34 patients with brucellosis in the Affiliated Hospital of Qingdao University from April 2010 to November 2014.Results Among the 34 patients,24 were male and 10 were female,aged from 12 to 71.The main clinical manifestations included fever,diaphoresis and arthralgia,and merged multiple organ symptoms.After cultured for 3 to 5 days,16 blood samples were positive,6 tissue samples were positive,with 2 positive in both samples.Thirty-four cases were identified Brucella species.Besides liver parameters abnormality and anemia,elevation of C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) were the most common laboratory findings.Patients got better prognosis after antibiotic combination therapy.Conclusions With increasing numbers of brucellosis cases in non-endemic areas,we should pay attention to bacteriological culture and other confirmation tests.On the other hand,techniques such as morphology and growth characteristics of Brucella should be mastered to prevent laboratory infection.