摘要目的 探讨DNA条形码技术在甘肃省啮齿动物鉴定中的应用,为建立甘肃省啮齿动物DNA条形码数据库提供资料.方法 选取甘肃省鼠类肝脏或肌肉作为研究对象,运用DNA条形码技术检测动物标本线粒体细胞色素C氧化酶Ⅰ亚基(CO Ⅰ)基因序列.结果 共检测54份样本,种内遗传距离为0～2％,种间遗传距离为18％ ～ 30％;邻接法系统树能区分8个物种的类群.结论 DNA条形码技术可用于啮齿动物鉴定,对于甘肃省自然疫源性疾病防治有重要意义.

abstractsObjective To apply the DNA barcoding technology for identification of rodent animals and to establish a rodent animal DNA barcode database in Gansu Province.Methods A total of 54 rodent animals were detected.DNA barcoding technology was used to analyze the DNA mitochondrial cytochrome C oxidase subunit Ⅰ (CO Ⅰ) gene sequence in Gansu Province.Results The intra-specific genetic distance was 0-2％ while the interspecific distance ranged from 18％ to 30％.Eight major clusters were apparently showed on a Neighbor joining tree.Conclusion DNA barcoding technology could overcome the shortcomings of the morphological identification,so it could be used to identify the rodent animals and has important implications for disease control and prevention in the natural focus of Gansu Province.