摘要目的 建立应用砷铈催化分光光度法进行尿中碘测量的不确定度评估方案,评定测定结果的质量和主要影响因素.方法 根据测定方法建立数学模型,评估碘酸钾基准试药、标准曲线校准、玻璃器材、天平称量、控温仪器等各因素的不确定度分量,并对尿碘标准物质检测结果的不确定度进行分析.结果 尿碘标准物质GBW09108i的碘含量为(72.6±3.7)μg/L,相对扩展不确定度为5.10％,在标准物质证书允许的误差范围内(10.0％).其中,标准曲线校准、玻璃器材及相关耗材体积的不确定度对扩展不确定度的影响较大.结论 建立的新方案适用于尿中碘的砷铈催化分光光度法的测量不确定度评估.

abstractsObjective To set up an uncertainty assessment method for urinary iodine measurement by As3+-Ce4+ catalytic spectrophotometry,in order to assess the accuracy of this method and find out the major influence factors.Methods A mathematical model was established based on the determination method to evaluate the uncertainty component of various factors such as potassium iodate benchmark,standard curve calibration,glass equipment,balance weighing,temperature control instrument,etc.,and the uncertainty of the test results of urinary iodine standard substances was analyzed.Results The iodine content of the urinary iodine standard material GBW09108i was (72.6 ± 3.7) μg/L,and the relative expansion uncertainty was 5.10％,within the tolerance range allowed by the reference material certificate (10.0％).Among them,the calibration of the standard curve,the uncertainty of the volume of the glass equipment and related consumables had a great influence on the extended uncertainty.Conclusion The method can be used to assess the uncertainty for determination of iodine in urine by As3+-Ce4+ catalytic spectrophotometry.