摘要目的 分析河北省布鲁菌病(简称布病)的流行情况及分离菌株的基因分型特征,为综合防治布病提供科学依据.方法 河北省2016年的布病疫情资料来源于中国疾病监测信息报告管理系统中的传染病报告信息管理系统,对数据进行描述流行病学分析(主要包括地区、人群、时间分布特征);河北省疾病预防控制中心布病实验室对布病疑似病例进行血培养,分离获得布鲁菌,采用多位点可变数目串联重复序列分析(MLVA)方法分析其基因分型特征.结果 2016年全省共报告布病病例3 774例,11个市均有病例报告,主要分布在张家口、承德和唐山市,且95.98％(167/174)的县有布病报告;职业以从事养殖和畜产品加工的农民和牧民为主,占90.22％(3 405/3 774);性别以男性为主,占74.38％(2 807/3 774);发病时间集中在1-8月,占83.76％(3 161/3 774).分离获得21株布鲁菌,20株为羊种3型布鲁菌,1株为牛种3型布鲁菌.MLVA结果显示,20株羊种菌Panel 1 (MLVA-8)基因型为42、43和114,其中42基因型占85.00％(17/20);Panel 1+Panel2A (MLVA-11)基因型为116、122和291,116基因型占85.00％ (17/20),均属于“东地中海组”.1株牛种菌Panel 1 (MLVA-8)基因型为36,Panel 1+Panel 2A(MLVA-11)基因型为72,属于“牛种C组”.结论 河北省布病疫情仍较严重,病原菌主要为羊种菌.MLVA作为布鲁菌基因分型鉴定的一种快速、可靠的方法,可为布病传染源的追溯提供依据.

abstractsObjective To analyze the brucellosis epidemic and genotyping characteristics of Brucella strains isolated from Hebei Province,and to provide scientific basis for brucellosis control and prevention.Methods Descriptive epidemiological analysis (including region,population,and time distribution characteristics) was performed using individual data of human brucellosis in Hebei Province from the National Notifiable Infectious Disease Reporting Information System of China Information System for Disease Control and Prevention in 2016,Brucella strains were isolated by phase culture bottles in Brucellosis Lab from Hebei Provincial Center for Disease Control and Prevention.Multiple-locus variable-number tandem-repeat analysis (MLVA) was used to identify the genotyping characteristics of the isolates.Results There were 3 774 reported cases in 2016,and 11 cities in the province had reported the cases.The cases were found mainly in Zhangjiakou,Chengde and Tangshan.And 95.98％ (167/174) of all counties were reported brucellosis cases.Vocational high risk population was farmers and herdsmen engaged in livestock and processing of animal products,accounting for 90.22％ (3 405/3 774).Male had the highest incidence,accounting for 74.38％ (2 807/3 774).The onset time was concentrated in January-August,accounting for 83.67％ (3 161/3 774).Twenty-one strains of Brucella were isolated,twenty isolates were Brucella melitensis biovar 3,and one isolate was Brucella abortus biovar 3.Panel 1 (MLVA-8) identified three genotypes 42,43,and 114,with genotype 42 representing 85.00％ (17/20);Panel 1 + Panel 2A (MLVA-11) identified three genotypes 116,122,291,with genotype 116 representing 85.00％ (17/20),which were identified as belonging to the East Mediterranean group.Panel 1 (MLVA-8) identified the genotype 36,Panel 1 + Panel 2A (MLVA-11) identified the genotype 72 of one Brucella abortus strain.It was identified as belonging to abortus C.Conclusions The brucellosis epidemic in Hebei is relatively severe,the pathogenic bacteria is mainly Brucella melitensis.The MLVA assay is a very practical and important molecular genotyping tool,it provides a basis for tracing the source of brucellosis infection.