摘要目的 观察青海省成人大骨节病患者血清炎性因子肿瘤坏死因子α(TNF-α)的变化,为科学防治大骨节病提供依据.方法 2017年7月,采用病例-对照研究方法,根据《大骨节病诊断》标准,在青海省海南藏族自治州兴海、贵德县大骨节病病区,选取20岁以上成人大骨节病患者104名[年龄(47.61±12.72)岁,男性50名、女性54名]、病区内健康对照95名[年龄(48.28±14.87)岁,男性35名、女性60名],同时在非病区循化县选取病区外健康对照73名[年龄(51.88±13.93)岁,男性35名、女性38名].采集观察人群空腹肘静脉血2ml,酶联免疫吸附试验(ELISA)法检测血清TNF-α.结果 3组人群年龄间比较差异无统计学意义(F=2.236,P＞ 0.05),性别间比较差异无统计学意义(x2=3.135,P＞ 0.05).大骨节病组患者血清TNF-α含量为(25.14±10.29)ng/L,病区内、外对照组人群血清TNF-α含量分别为(21.84±9.37)、(24.98±8.98)ng/L,组间比较差异有统计学意义(F=3.486,P＜ 0.05),其中大骨节病组、病区外对照组血清TNF-α含量均高于病区内对照组(P均＜ 0.05),大骨节病组与病区外对照组间比较差异无统计学意义(P＞0.05).结论 青海省大骨节病病区的高原成人大骨节病患者可能存在炎性改变.

abstractsObjective To understand the changes of serum tumor necrosis factor-α (TNF-α) among adults with Kaschin-Beck disease (KBD) in Qinghai,and to provide scientific data for KBD prevention and control.Methods A case-control study was used,according to "Diagnosis of Kaschin-Beck disease",104 cases of KBD patients [aged (47.61 ± 12.72) years old;50 males,54 females] and 95 cases of internal healthy controls [aged (48.28 ±14.87) years old;35 males,60 females],which were from KBD areas in Xinghai and Guide counties of Hainan Tibetan Autonomous Prefecture,Qinghai Province,and 73 cases of external healthy controls [aged (51.88 ± 13.93)years old;35 males,38 females] from non-KBD region of Xunhua County were included in July 2017.Serum TNF-α was determined via the enzyme linked immunosorbent assay (ELISA) method.Results Totally 272 persons were studied and divided into three groups,there was no statistical significant difference of ages among the three groups (F =2.236,P ＞ 0.05),meanwhile,there was no statistical significant difference of genders among the three groups (x2 =3.135,P ＞ 0.05).The serum TNF-α levels of KBD patients,internal and external healthy controls were (25.14 ± 10.29),(21.84 ± 9.37),and (24.98 ± 8.98) ng/L,respectively,which were statistically significantly different among the three groups (F =3.486,P ＜ 0.05).The serum TNF-α levels in the KBD group and the external healthy control were higher than that in internal healthy control (P ＜ 0.05).There was no significant difference between the KBD group and the external healthy control (P ＞ 0.05).Conclusion There may exist inflammatory changes among KBD patients in KBD areas of Qinghai Province.