摘要目的 了解贵阳市致倦库蚊成蚊对4种常用杀虫剂的抗药性水平.方法 2015年8-9月,用幼虫勺捕法采集贵阳市8个地区的野外致倦库蚊幼虫,在实验室饲养至成蚊.采用接触筒法测定成蚊对4种常用杀虫剂的抗药性,计算1h击倒率和恢复24h死亡率.根据死亡率判断抗药性水平:＜90％为抗性群体(R);90％ ～＜ 98％为可能抗性群体(M);≥98％为敏感群体(S).结果 将致倦库蚊成蚊分别暴露于7.70 g/L敌敌畏、3.30 g/L残杀威、0.25 g/L高效氯氰菊酯和0.20 g/L溴氰菊酯,1h击倒率范围分别为57.78％(52/90)～91.11％(82/90)、86.67％(78/90) ～ 100.00％(90/90)、23.33％(21/90) ～ 77.78％(70/90)和27.78％(25/90) ～ 88.89％(80/90);恢复24 h死亡率范围分别为61.11％(55/90) ～ 94.44％(85/90)、90.00％(81/90) ～ 97.78％(88/90)、23.33％(21/90) ～ 73.33％(66/90)和21.11％(19/90) ～ 72.22％(65/90).结论 贵阳市致倦库蚊成蚊对4种常用杀虫剂均产生了一定程度的抗药性,其中对高效氯氰菊酯、溴氰菊酯等拟除虫菊酯类杀虫剂的抗药性较高.

abstractsObjective To investigate the insecticide resistance levels of adult Culex pipiens quinquefasciatus to four common insecticides in Guiyang City.Methods Larvae of Culex pipiens quinquefasciatus were collected from 8 areas of Guiyang City by larval scoop method from August to September 2015,and raised in the laboratory to adult Culex pipiens quinquefasciatus.Contact tube method was used to determine the insecticide resistance levels of adult Culex pipiens quinquefasciatus to four common insecticides,and knockdown rate of 1 h and mortality rate of 24 h recovery were calculated.The resistance level was judged according to the mortality rate:＜ 90％ was in the resistant group (R);90％-＜ 98％ was in the potential resistant group (M);and ≥98％ was in the sensitive group (S).Results The adult Culex pipiens quinquefasciatus were exposed to 7.70 g/L dichlorvos,3.30 g/L propoxur,0.25 g/L beta-cypermethrin and 0.20 g/L dehamethrin,knockdown rates of 1 h were 57.78％ (52/90)-91.11％ (82/90),86.67％ (78/90)-100.00％ (90/90),23.33％ (21/90)-77.78％ (70/90) and 27.78％ (25/90)-88.89％ (80/90),respectively;and mortality rates of 24 h recovery were 61.11％ (55/90)-94.44％ (85/90),90.00％ (81/90)-97.78％ (88/90),23.33％ (21/90)-73.33％ (66/90) and 21.11％ (19/90)-72.22％ (65/90),respectively.Conclusion Adult Culex pipiens quinquefasciatus in Guiyang City have developed some resistance to four common insecticides,in which the mosquito has a higher resistance to pyrethroid insecticides such as beta-cypermethrin and deltamethrin.