摘要目的:探讨氟化钠干预下骨钙素（BGP）对仔鼠骨质生长发育和相关激素表达水平的影响。方法:选取清洁级雌性和雄性SD大鼠各24只，体质量为180 ~ 220 g，将大鼠按雌雄1∶1同笼交配，连续10 d。采用饮水染氟法建立氟中毒大鼠模型，按体质量采用随机数字表法将雌鼠分为3组，每组8只，分别为高剂量、低剂量和对照组，饮水中氟化钠含量分别为200、100、0 mg/L。母鼠染氟时间为妊娠第0天至仔鼠出生后第3周（断乳前）。断乳后，每组选取10只雄性仔鼠，继续以相同含量和方式染氟至出生后第12周。仔鼠断乳前每周和断乳后每2周测量体质量、身长。采用酶联免疫吸附测定法（ELISA）检测染氟12周各组仔鼠血清BGP、甲状旁腺激素（PTH）、降钙素（CT）、碱性磷酸酶（ALP）含量。结果:仔鼠染氟第2周，低、高剂量组体质量[（27.25 ± 3.57）、（26.27 ± 4.48）g]、身长[（6.92 ± 0.46）、（6.50 ± 0.54）cm]低于对照组[（31.32 ± 3.62）g、（7.19 ± 0.26）cm， P均< 0.05]，但高剂量组和低剂量组体质量、身长比较差异无统计学意义（ P均> 0.05）；染氟第3周起，高剂量组体质量、身长低于低剂量组和对照组（ P均< 0.05）。对照组血清BGP、PTH、ALP含量[（5.42 ± 0.26）mg/L、（157.53 ± 32.21）ng/L、（36.62 ± 6.01）U/L]低于低剂量组[（6.15 ± 0.29）mg/L、（212.26 ± 51.97）ng/L、（50.68 ± 6.11）U/L]和高剂量组[（7.31 ± 0.77）mg/L、（274.21 ± 60.32）ng/L、（74.99 ± 9.08）U/L]，CT含量[（182.40 ± 17.39）ng/L]高于低、高剂量组[（135.77 ± 14.06）、（70.09 ± 13.49）ng/L]，差异有统计学意义（ P均< 0.05）；高剂量组血清BGP、PTH、ALP含量高于低剂量组，CT含量低于低剂量组（ P均< 0.05）。 结论:氟化钠可能通过促进BGP分泌，参与调节相关激素表达水平，从而影响仔鼠骨质生长发育。

abstractsObjective:To investigate the effects of osteocalcin (BGP) on bone growth and development and the expression levels of related hormones in offspring rats under the intervention of sodium fluoride.Methods:Twenty-four clean female SD rats and 24 clean male SD rats were selected, weighing 180-220 g, and the rats were mated in a 1∶1 cage for 10 d. The fluorosis rat model was established by drinking the fluorosis water method, female rats were divided into 3 groups according to body weight by random number table method, each group of 8 rats, including the high-dose, low-dose and control groups, with sodium fluoride of 200, 100, 0 mg/L in drinking water. The female rats were exposed to fluoride from the 0th day of pregnancy to the 3rd week after the offspring rats were born (before weaning). After weaning, 10 male offspring rats were selected from each group and continued to be exposed to fluoride in the same amount and manner until the 12th week after birth. The body weight and length of the offspring rats were measured every week before weaning and every two weeks after weaning. Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum BGP, parathyroid hormone (PTH), calcitonin (CT), and alkaline phosphatase (ALP) contents in the offspring rats of each group exposed to fluoride for 12 weeks.Results:In the 2nd week of fluoride exposure, the body weights [(27.25 ± 3.57), (26.27 ± 4.48) g] and body lengths [(6.92 ± 0.46), (6.50 ± 0.54) cm] of the low-dose and high-dose groups were lower than those of the control group [(31.32 ± 3.62) g, (7.19 ± 0.26) cm, P < 0.05], but there were no significant differences in body weights and lengths between the high-dose group and the low-dose group ( P > 0.05). From the 3rd week of fluoride exposure, the body weight and length of the high-dose group were lower than those of the low-dose group and the control group ( P < 0.05). Serum BGP, PTH and ALP contents [(5.42 ± 0.26) mg/L, (157.53 ± 32.21) ng/L, (36.62 ± 6.01) U/L] in the control group were lower than those of the low-dose group [(6.15 ± 0.29) mg/L, (212.26 ± 51.97) ng/L, (50.68 ± 6.11) U/L] and high-dose group [(7.31 ± 0.77) mg/L, (274.21 ± 60.32) ng/L, (74.99 ± 9.08) U/L], and CT content [(182.40 ± 17.39) ng/L] was higher than those of the low-dose and high-dose groups [(135.77 ± 14.06), (70.09 ± 13.49) ng/L], and the differences were statistically significant ( P < 0.05); serum BGP, PTH and ALP contents in the high-dose group were higher than those in the low-dose group, and the CT content was lower than that in the low-dose group ( P < 0.05). Conclusion:Sodium fluoride may be involved in regulating the expression of related hormones by promoting the secretion of BGP, thereby affecting the bone growth and development of offspring rats.