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High performance liquid chromatography with immobilized Ru(bpy)32+-KMn04 chemiluminescence detection and its application in metabolism of repaglinide in pig liver microsomes

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摘要A novel high performance liquid chromatography-chemiluminescence (HPLC-CL) method for investigation of in vitro metabolism of repaglinide in pig liver microsomes with microdialysis sampling technique was developed. The chromatographic separation was performed on a Hypersil BDS-C18 column with an isocratic mobile phase consisting of methanol and 0.01 mol/L KH2PO4 (pH 3.0) (volume ratio 75:25) at a flow rate of 1.0 mL/min. The detection was based on the chemiluminescence reaction of repaglinide with acidic potassium permanganate (KMnO4) and tris (2,2'-bipyridyl)ruthenium(III) (Ru(bpy)33+), which was immobilized on the cationic ion-exchange resin for obtaining high sensitivity and reducing consumption of expensive reagent.

作者单位 Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, China ^[1]

关键词 High performance liquid chromatography Chemiluminescence Microdialysis Repaglinide Immobilized Ru(bpy)32+

分类号 R151（营养卫生、食品卫生）

DOI 10.1016/j.cclet.2011.05.023

发布时间 2012-06-29（万方平台首次上网日期，不代表论文的发表时间）

基金项目

This work was supported financially by the Fundamental Research Funds for the Central Universities （(GK20091004)）

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