摘要目的 探讨吸烟对肺表面活性蛋白A(SP-A)和肺表面活性蛋白D(SP-D)含量的影响.方法 通过双抗体夹心ELISA法测定11名吸烟者和13非吸烟者肺泡灌洗液中的SP-A和SP-D的含量.结果 非吸烟者肺泡灌洗液中SP-A和SP-D含量均明显高于吸烟者[(3.1±0.40)μg/ml与(1.8±0.4)μg/ml,(1.5±0.2)μg/ml与(0.6±0.1)μg/ml,均P<0.05].结论 吸烟者SP-A和SP-D水平的降低可能损害宿主的肺泡免疫功能,而且可能对慢性阻塞性肺疾病的产生起重要作用.

abstractsObjective To observe whether cigarette smoke alter the component of pulmonary surfactant pro-tein A and D. Methods In this study, we determined the contents of SP-A and SP-D in BAL fluids of healthy smok-ers and nonsmokers by enzyme linked immunosorbent assay(ELISA). Resluts The contents of SP-A and SP-D in BAL fluids were significantly decreased in smokers compared to those in nonsmokers[ (3.1±0.40) μg/ml vs (1.8± 0.4)μg/ml, (1.5±0.2) μg/ml vs (0.6±0.1)μg/m, all P <0.05]. Conclusion These results suggested that the decreased levels of SP-A and SP-D in smokers may impair the host defense functions of suffactant in the peripheral airways and might have a crucial role in the development of chronic obstructive lung disease.