摘要目的 探讨ER、PR、C-erbB-2、CylinD1、Rb在乳腺不同病变组织中的表达及意义.方法 免疫组织化学法检测ER、PR和C-erbB-2、CylinD1、Rb蛋白在正常乳腺组织、单纯性增生、不典型增生和早期癌中的表达.结果 ER、PR在乳腺从正常组织到癌变过程中阳性表达率逐渐递增,ER、PR与乳腺增生病情程度密切相关,阳性表达率在不典型增生各组分别为63.2%和68.4%,显著高于正常乳腺组(分别为31.3%和37.5%)和单纯增生组(分别为37.1%和42.9%).CylinD1、C-erbB2在癌前不同病变中的阳性表达率也显逐渐递增.Rb蛋白阴性表达早期乳腺癌最高,正常乳腺组织、单纯性增生为0.结论 ER、PR、C-erbB-2、CylinD1的高表达和Rb蛋白的低表达与乳腺癌变相关.

abstractsObjective To explore the expression and significance of ER, PR,C-erbB-2,CylinDl, Rb in different precancerous lesions of breast. Methods The expressions of ER, PR, C-erbB-2, CylinD1, Rb in breast normal tissue,hyperplasia, atypical hyperplasia and early-stage breast cancer were examined by immunohistochemical technique. Results The expressions of ER and PR were increasing time trend in the different precancerous lesions, and were related to the breast proliferative degree; the positive expression rates of ER and PR in atypical hyperplasia were 63.2% and 68. 4% respectively,significantly higher than those in normal breast tissue(31.3% and 37. 5% respectively) and in hyperplasia(37. 1% and 42.9% respectively). The positive expression rates of CylinD1 and C-erbB-2 were increasing time trend in the different precancerous lesions. The negative expression rate of Rb was the highest in early-stage breast cancer,and it was zero in normal breast tissue and hyperplasia. Conclusion The five tumor markers are important factors in breast carcinogenesis, and the overexpression of ER, PR, C-erbB-2, CylinD1 and Rb protein low-expression may be one of the causes of breast neoplasms.