摘要目的 探讨血小板相关抗体对血小板输注效果的影响.方法 采用酶联免疫吸附试验(ELISA)检测120例长期反复输血并且需要输注血小板患者的血小板相关抗体,检测输血前后血小板计数,采用血小板计数增值考察血小板输注效果.采用简易致敏红细胞血小板血清学技术( SEPSA)对血小板相关抗体阳性患者进行血小板交叉配型后进行血小板输注.结果 反复输血的患者中血小板相关抗体阳性率为65.0％,血小板输注的有效率为61.7％.血小板相关抗体阳性患者和阴性患者输注血小板时输注无效率分别为84.6％和19.0％,差异有统计学意义(x2=46.914,P＜0.01).对血小板相关抗体阳性患者采用SEPSA进行血小板配型后输注血小板有效率为85.9％.结论 血小板相关抗体阳性与血小板输注无效相关,血小板配型可有效提高临床输注效果.

abstractsObjective To investigate effect of platelet-associated antibody to the platelet transfusion.Methods Platelet-associated antibody in 120 patients who needed repeated platelet transfusion for long-time was determined by enzyme-linked immunosorbent assay (ELISA),and platelet count was assayed before and after blood transfusion,and the effect of platelet transfusion was investigated by corrected platelet count.Crossmatch approcah was used to screen compatible platelet for patients with platelet relative antibody positive.Results The ratio of positive platelet-aasociated antibody in patients who needed repeated platelet transfusion was 65.0％,and the ratio of platelet transfusion resistance was 61.7％.The ratios of platelet transfusion resistance were 84.6％ and 19.0％ respectively in patients with positive platelet-associated antibody and in patients with negative platelet-associated antibody,which were different in statistic in both groups( x2 =,46.914,P ＜0.0l ).The effective ratio of platelet transfusion in patients with positive platelet-associated antibody after crossmatch approach was 85.9％.Conclusion Positive platelet-associated antibody was related to platelet transfusion resistance with high risk,and crossmatch approach could increase the effect of platelet transfusion.