摘要目的 探讨乙型肝炎病毒外膜大蛋白(HBV-LP)与乙型肝炎病毒脱氧核糖核酸(HBV-DNA)的相关性,动态检测两项指标,分析其在诊断乙型肝炎和临床治疗中的意义.方法 采用酶联免疫吸附试验和实时荧光定量聚合酶链反应技术对230例乙型肝炎患者血清标本进行HBV-LP和HBV-DNA平行检测.结果 乙型肝炎病毒感染患者血清HBV-LP含量与HBV-DNA拷贝数间呈正相关(r=0.84,P＜0.01),230例乙肝病毒感染患者血清标本中,HBV-LP阳性率为84.78％,HBV-DNA阳性率为84.35％,两者差异无统计学意义(P＞0.05).55例HBeAg阴性血清标本中,HBV-LP与HBV-DNA的阳性检出率分别为63.63％(35/55)、58.18％(32/55),两者差异无统计学意义(P＞0.05).HBV-DNA阳性194例中,HBV-LP阳性率(82.47％)明显高于HBeAg阳性率(52.06％)(x2=80.3,P＜0.01).结论 HBV-LP及HBV-DNA是判断乙型肝炎病毒感染患者体内病毒复制程度的重要指标,尤其是HBeAg阴性患者体内病毒复制及预后判断有价值的血清学检测指标,为临床诊断及治疗提供可靠的实验室数据.

abstractsObjective To explore the correlation between hepatitis B virus large surface protein(HBV-LP)and hepatitis B virus deoxyribonucleic acid(HBV-DNA),analyze the clinical significance of detecting the two index dynamically for diagnosis and treatment of hepatitis B.Methods Enzyme linked immunosorbent assay(ELISA)and fluorescent quantitation polymerase chain reaction(FQ-PCR)were used to detect the levels of HBV-LP and HBV-DNA in serum specimen of 230 hepatitis B patients.Results There was a positive correlation between the content of HBV-LP and copy numbers of HBV-DNA in serum of hepatitis B positive cases(r=0.84,P＜0.01).The positive rate was 84.78％ in HBV-LP and 84.35％ in HBV-DNA of 230 HBV positive cases.As a result there was no significant difference(P＞0.05)in this study.Therefore,the positive rate was 63.63 ％(35/55)in HBV-LP and 58.18％(32/55)in HBV-DNA of 55 HBV negative cases.There was also no significant difference(P＞0.05)in this study.However,the positive rate of HBV-LP(82.47％)was higher than HBE(52.06％)in the 194 HBV-DNA positive cases.There was significantly different(P＜0.01).Conclusion HBV-LP and HBV-DNA are the significant index of the degree of hepatitis B virus replication in hepatitis B positive cases,especially in determining the virus replication and prognosis of treatment in HBe Ag negative,providing the reliable laboratory data for the clinical diagnosis and treatment.