摘要目的：用三种血清学方法同时对布鲁菌病高危人群血清学进行检测，比较不同方法的优劣，为布鲁菌检测提供合适的检测技术。方法按照《布鲁菌病监测标准》（GB 16885-1997）和《布鲁菌病诊断标准》（WS269-2007）中规定方法，分别用虎红平板凝集试验（RBPT）、试管凝集试验（SAT）、酶联免疫吸附法（ELISA）对安徽省涡阳县某羊场布鲁菌病高危人群血清进行检测。结果 RBPT、SAT 和 ELISA 阳性率分别为19．1％、12．1％、16．3％，与 SAT 相比，RBPT 的敏感度、特异度、准确率、ROC 曲线下面积分别为88．5％、91．8％、91．4％、0．81；ELISA 的敏感度、特异度、准确率、和 ROC 曲线下面积为：93．9％、95．1％、94．9％、0．88。结论 ELISA 检测方法敏感性、特异性、准确率和 ROC 曲线下面积均高于其他方法，可在实际检测工作中推广。选择适宜的布鲁菌病检测方法，制定布鲁菌病监测和技术策略，可以有效地控制布鲁菌病的发生和流行。

abstractsObjective To detect serum brucellosis in high risk population,three methods were used and their advantages and disadvantages was compared.Methods In accordance with the "surveillance standard for brucello-sis"(GB 16885 -1997)and "brucellosis diagnostic criteria"(WS269 -2007)in the prescribed method,rose -bengal plate agglutination test (RBPT),standard -tube agglutination test (SAT)and enzyme linked immunoassay assay (ELISA)were used to detect brucellosis and analysis of its diagnostic significance in the high risk population of sheep farm of Guoyang county in Anhui province.Results The positive rates of RBPT,SAT and ELISA were 19.1%,12.1% and 16.3% in 257 blood samples,respectively.Compared to SAT,the sensitivity,specificity,accura-cy and the area under the ROC curve of RBPT were 88.5%,91.8%,91.4%,0.81,respectively,which of ELISA were 93.9%,95.1%,94.9%,0.88.Conclusion The sensitivity,specificity,accuracy and the area under the ROC curve of ELISA were higher than those of other methods.Proper method,early surveillance and effective technology can help to control the occurrence and epidemic of brucellosis in the actual test work promotion.