摘要目的：通过检测腺病毒肺炎患儿血清和支气管肺泡灌洗液中白细胞介素6、白细胞介素10及肿瘤坏死因子α的表达，并且与健康对照组相比较，探讨其与儿童腺病毒肺炎临床表现的相关性。方法采用酶联免疫吸附试验（ELISA）法检测40例腺病毒肺炎患儿及20例健康对照儿童血清和支气管肺泡灌洗液中白细胞介素6、白细胞介素10及肿瘤坏死因子α水平。结果腺病毒肺炎急性期和恢复期的患儿外周血中 IL-6、IL-10、TNF-α的水平均明显高于对照组，差异均有统计学意义（均 P ＜0．05）。腺病毒肺炎急性期患儿外周血中 IL-6、IL-10、TNF-α的水平明显高于恢复期[（47．5±17．7）pg／mL 比（22．3±6．5）pg／mL，（7．5±1．3）pg／mL 比（5．3±2．1）pg／mL，（123．4±5．3）pg／mL 比（71．3±5．4）pg／mL]，差异均有统计学意义（t ＝7．57、8．39、11．57，均 P ＜0．05）。结论腺病毒肺炎患儿外周血以及支气管肺泡灌洗液中白细胞介素6、白细胞介素10及肿瘤坏死因子α水平显著性升高，表明白细胞介素6、白细胞介素10及肿瘤坏死因子α可能在儿童腺病毒肺炎的发病中起着重要的作用。

abstractsObjective To detect the levels of interleukin -6(IL -6),interleukin -10(IL -10)and tumor necrosis factor -α(TNF -α)in serum and broncholveolar lavage fluid of children with adenovirus pneumonia,and compared with the healthy control group,and to explore the correlation with the clinical manifestations of adenovirus pneumonia.Methods Enzyme linked immunosorbent assay(ELISA)was used to detect IL -6,IL -10 and TNF -αlevels in serum and bronchoalveolar lavage fluid of 40 children with adenovirus pneumonia and 20 healthy control chil-dren.Results The levels of IL -6,IL -10 and TNF -αin serum and bronchoalveolar lavage fluid of 40 children with adenovirus pneumonia were significantly higher than the control subjects(all P <0.05).In addition,the levels of IL -6,IL -10 and TNF -αin serum and bronchoalveolar lavage fluid of children in the acute phase were significantly higher than children in the recovery phase[(47.5 ±17.7)pg/mL vs.(22.3 ±6.5)pg/mL;(7.5 ±1.3)pg/mL vs. (5.3 ±2.1)pg/mL;(123.4 ±5.3)pg/mL vs.(71.3 ±5.4)pg/mL],the differences were statistically significant (t =7.57,8.39,11.57,all P <0.05).Conclusion The levels of IL -6,IL -10 and TNF -αin peripheral blood and bronchoalveolar lavage fluid of children with adenovirus pneumonia increased significantly,suggest that IL -6, IL -10 and TNF -αmay play important roles in the incidence of adenovirus pneumonia in children.