摘要目的 探讨天山堇菜提取物的降糖效果.方法 以总黄酮为指标,通过正交设计考察提取条件,回流提取法对天山堇菜进行提取.链脲佐菌素(STZ)建立小鼠糖尿病模型,再利用天山堇菜提取物进行降糖实验,将小鼠分为高剂量组,中剂量组,低剂量组,空白对照组,阳性对照组及正常小鼠组,每组15只,观察不同分组小鼠血糖和体质量等变化情况.结果 天山堇菜提取物高剂量组可降低糖尿病小鼠的血糖水平,平均血糖水平由(18.4±5.8)mmol/L降至(11.6±3.4)mmol/L,提取物中剂量组可降低糖尿病小鼠的降糖水平,平均血糖水平由(24.8±5.6)mmol/L降至(18.5±4.6)mmol/L,差异均有统计学意义(均P<0.05).结论 天山堇菜提取物对糖尿病小鼠血糖有一定的降低作用.

abstractsObjective To explore the hypoglycemic effects of Viola tianshanica Maxim.Methods Extraction conditions by orthogonal design research,in total flavonoids as index by reflux extraction for Viola tianshanica Maxim extracted.Established mouse model of diabetes with streptozotocin(STZ),reused Viola tianshanica Maxim extract for hypoglycemic experiment.The mice were divided into high dose group,middle dosage group,low dose group,blank control group,positive control group and normal mice group,15 mice in each group.The mice blood glucose and body weight of the different groups were observed.Results Viola tianshanica Maxim in a high concentration had significantly hypoglycemic effect in diabetic mice,average blood glucose level decreased from (18.4±5.8)mmol/L to (11.6±3.4)mmol/L,and the concentration of the extract could reduce the level of blood glucose in diabetic mice,average blood glucose level decreased from (24.8±5.6)mmol/L to (18.5±4.6)mmol/L,the differences were statistically significant(all P<0.05).Conclusion Viola tianshanica Maxim crude extract has a certain hypoglycemic effect.