摘要Salivary gland-specific transcriptomes of nine heteropteran insects with distinct feeding strategies(predaceous,hematophagous,and phytophagous)were analyzed and annotated to compare and identify the venom components as well as their expres-sion profiles.The transcriptional abundance of venom genes was verified via quantita-tive real-time PCR.Hierarchical clustering of 30 representative differentially expressed venom genes from the nine heteropteran species revealed unique groups of salivary gland-specific genes depending on their feeding strategy.The commonly transcribed genes in-cluded a paralytic neurotoxin(arginine kinase),digestive enzymes(cathepsin and serine protease),an anti-inflammatory protein(cystatin),hexamerin,and an odorant binding pro-tein.Both predaceous and hematophagous(bed bug)heteropteran species showed rela-tively higher transcription levels of genes encoding proteins involved in proteolysis and cytolysis,whereas phytophagous heteropterans exhibited little or no expression of these genes,but had a high expression of vitellogenin,a multifunctional allergen.Saliva pro-teomes from four representative species were also analyzed.All venom proteins iden-tified via saliva proteome analysis were annotated using salivary gland transcriptome data.The proteomic expression profiles of venom proteins were in good agreement with the salivary gland-specific transcriptomic profiles.Our results indicate that profiling of the salivary gland transcriptome provides important information on the composition and evolutionary features of venoms depending on their feeding strategy.