摘要Aphids are important agricultural pests,vectors of many plant viruses and have sophisticated relationships with symbiotic microorganisms.Abundant asymptomatic RNA viruses have been reported in aphids due to the application of RNA-seq,but aphid-virus interactions remain unclear.Bunyavirales is the most abundant RNA virus order,which can infect mammals,arthropods,and plants.However,many bunyaviruses have specific hosts,such as insects.Here,we discovered 18 viruses from 10 aphid species by RNA-seq.Importantly,a widespread presence bunyavirus,Aphid bunyavirus 1(ABV-1),was determined to have a wide host range,infecting and replicating in all 10 tested aphid species.ABV-1 may be transmitted horizontally during feeding on plant leaves and verti-cally through reproduction.In a comparison of the physiological parameters of ABV-lhigh and ABV-1low strains of pea aphid,higher ABV-1 titers reduced the total nymphal du-ration and induced the reproduction.Moreover,viral titer significantly affected the lipid and protein contents in pea aphids.In summary,we proposed that ABV-1 may have sta-ble symbiont-like relationships with aphids,and these observations may provide a new direction for studying bunyaviruses in aphids and establishing a model for virus-aphid interactions.