摘要维生素D缺乏与多种疾病相关,血清25(OH)D水平可以作为维生素D营养状况的客观指标.液相色谱质谱法(LC-MS)是临床营养常规测量25(OH)D的标准方法.本文总结了检测25 (OH) D2和25 (OH)D3的意义,比较了各种25(OH)D的检测方法及其优缺点,介绍了我国采用美国国立标准技术研究院质控品标准的LC-MS法常规检测25(OH)D2和25(OH)D3的情况.

abstractsMany diseases are associated with vitamin D deficiency.Serum 25 (OH)D levels can serve as objective indicators of the nutritional status of vitamin D.Liquid chromatography-mass spectrometry (LC-MS) method may be applied for routine measurement of 25 ( OH ) D levels in clinical practice.This article reviewed the significance of detecting 25 (OH) D2 and 25 (OH) D3,compared the advantages and disadvantages of different 25 (OH) D measurement methods,and introduced the application of LC-MS method for routine detection of serum 25 (OH) D2 and 25 (OH) D3 levels following the standard by National Institute of Standards and Technology of America.