摘要目的 探讨MTNR1B基因DNA甲基化与妊娠期糖尿病及妊娠期间糖脂代谢指标的相关性.方法 纳入2009年至2012年孕中期行100克口服葡萄糖耐量试验(OGTT)的妊娠期糖尿病患者50例以及年龄和孕前体重指数(body mass index,BMI)匹配的正常糖耐量孕妇50例,收集临床指标及孕中期全血标本,完成MTNR1B基因DNA甲基化测定.结果 校正年龄与孕前BMI后,据翻译起始位点+64 bp的MTNR1B CpG位点DNA甲基化程度与妊娠期糖尿病相关(OR=0.859,95％可信区间:0.772～0.955,P=0.005),MTNR1B基因许多位点DNA甲基化水平和妊娠期间糖脂代谢指标相关.结论 MTNR1B基因DNA甲基化和妊娠期糖尿病与妊娠期糖脂代谢相关.

abstractsObjective To explore the relationship of MTNR1B DNA methylation with gestational diabetes and gestational glucose and lipid metabolism features.Methods 50 patients with gestational hyperglycemia,diagnosed by 100 g oral glucose tolerance test (OGTT) during mid-trimester were selected between 2009 and 2012.50 pregnant women with normal glucose tolkerance of matched age and body mass index were included in the control group.The blood samples during mid-trimester and the clinical parameters were collected.MTNR1B DNA methylation levels were measured.Results After adjusting age and body mass index,the CpG locus located at +64 bp away from the translation initiation site of MTNR1B was related with gestational diabetes (OR=0.859,95％ CI:0.772-0.955,P=0.005).DNA methylation level of several MTNR1B loci was also related with gestational glucose and lipid metabolism features.Conclusion MTNR1B DNA methylation is related with gestational diabetes and gestational glucose and lipid metabolism.