摘要Parental food provisioning is crucial for the growth and survival of offspring.Growth rate depends on food quality and food supplied to offspring may differ from what adults use for their own.In the case of steppe passerine birds,detailed characterization on nestling dietary composition,as well as prey choice and resource partitioning among species,is a pending subject.Dietary differences between nestlings and adults remain also largely unexplored.By using faecal DNA metabarcoding,we described the diet of nestlings and adults of five shrub-steppe passerine species over the 2017-2019 breeding seasons in central Spain.We also monitored arthropod availability in the field to assess dietary selection.We expected interspecific dietary differences to limit competition for food resources among sympatric species,as well as parental selection of high quality prey for nestlings.We also predicted age-related differences,with nestlings being fed nutrient-rich prey more frequently than adults.The main arthropod orders provisioned to nestlings were Orthoptera,Julida,Araneae and Lepi-doptera.Nestlings of the different species showed high interspecific diet overlap,indicating both a coincidence in growth needs among bird species and no or little limitation of the most profitable resources during the breeding season.Adults of all species showed higher diet richness than nestlings,and age-related differences in prey composition were mainly driven by the selection of the most easily digestible,larger protein-and calcium-rich prey for nestlings,which may favour their rapid growth,and avoiding highly sclerotized and less nutritional prey such as ants.Our study sheds light on the basic ecology and conservation of these declining steppe birds,indicating that interspecific competition may not be a major factor during the breeding season.Given the current global decline of arthropods,further long-term research would be necessary,along with the implementation of effective conservation measures that ensure a sufficient availability of resources identified as key prey in the diet of steppe bird nestlings.