摘要The prevalence of domestic and industrial electrical appliances has raised concerns about the health risk of extremely low-frequency magnetic fields (ELF-MFs).At present,the effects of ELF-MFs on the central nervous system are still highly controversial,and few studies have investigated its effects on cultured neurons.Here,we evaluated the biological effects of different patterns of ELF-MF exposure on primary cultured hippocampal neurons in terms of viability,apoptosis,genomic instability,and oxidative stress.The results showed that repeated exposure to 50-Hz 2-mT ELF-MF for 8 h per day after different times in culture decreased the viability and increased the production of intracellular reactive oxidative species in hippocampal neurons.The mechanism was potentially related to the up-regulation of Nox2 expression.Moreover,none of the repeated exposure patterns had significant effects on DNA damage,apoptosis,or autophagy,which suggested that ELF-MF exposure has no severe biological consequences in cultured hippocampal neurons.