摘要This experiment sought to observe the migration and distribution of bone mesenchymal stem cells transfected with the cytosine deaminase gene(BMSCs-CD/eGFP)after transplantation in vivo through three pathways.In addition,we examined the tropism of these cells to glioma.Intracranial C6 glioma models were established in Sprague-Dawley rats using an intracranial stereotacticinoculation method.When tumors were 7 days old,rats were inoculated with 1×106 BMSCs-CD/eGFP cells via the tumor-bearing internal carotid artery,the contralateral hemisphere and the tumor-bearing glioma.Fluorescence microscopy revealed that BMSCs-CD/eGFP exhibited a strong capacity for migration to tumors.BMSCs-CD/eGFP transplanted via the tumor-bearing internal carotid artery were observed to distribute in glioma tissues.BMSCs-CD/eGFP inoculated via the ipsilateral glioma mainly located within and at the edge of glioma tissues.BMSCs-CD/eGFP inoculated via the contralateral hemisphere mainly distributed at the proximal end of the tumor at the incubation site.