换一批Screening proteins that interact with mutant superoxide dismutase 1 from familial amyotrophic lateral sclerosis using a yeast two-hybrid system
摘要The present study screened a human fetal brain cDNA library to find the proteins that interact with mutant superoxide dismutase 1 (SOD1) using a yeast two-hybrid system. Using BLAST software, 15 real proteins which interacted with mutant SOD1 were obtained, including 8 known proteins (protein tyrosine-phosphatase non-receptor type 2, TBC1D4, protein kinase family, splicing factor, arginine/serine-rich 2, SRC protein tyrosine kinase Fyn, β-sarcoglycan; glycine receptor α2, microtubule associated protein/microtubule affinity-regulating kinase 1, ferritin H chain), and 7 unknown proteins. Results demonstrated interaction of mutant SOD1 with microtubule associated protein/microtubule affinity-regulating kinase 1 and β-sarcoglycan.
更多相关知识
关键词
yeast two-hybrid systemmutant superoxide dismutase 1cDNA libraryprotein-protein interaction screenamyotrophic lateral sclerosis
栏目名称
DOI
10.3969/j.issn.1673-5374.2011.26.002
发布时间
2011-11-17（万方平台首次上网日期，不代表论文的发表时间）
基金项目
the National Natural Science Foundation of China,（No.30300116）
- 浏览0
- 被引0
- 下载0
相似文献
- 中文期刊
- 外文期刊
- 学位论文
- 会议论文
