换一批Recombinant adeno-associated virus delivered human thioredoxin-PR39 prevents hypoxia-induced apoptosis of ECV304 cells
摘要Human thioredoxin and antibacterial peptide, PR39, have been shown to have potent antioxidant effects that may prolong survival of cells during hypoxia. The pSSCMV/human thioredoxin-PR39 vector was successfully constructed in this study and used to infect ECV304 cells. Transfected ECV304 cells were incubated at 1%, 5% hypoxic, and normal oxygen conditions. We found that the number of apoptotic cells after transfection with recombinant adeno-associated virus-human thioredoxin -PR39 was significantly lower than controls, suggesting a protective effect of the recombinant human thioredoxin-PR39 protein on hypoxic cells.
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关键词
human thioredoxinantimicrobial peptide PR39fusion generecombinant adeno-associated virusgene therapyapoptosishypoxia
分类号
R741（神经病学）
DOI
10.3969/j.issn.1673-5374.2012.09.012
发布时间
2012-05-07（万方平台首次上网日期，不代表论文的发表时间）
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