摘要Muscle-derived stem cells were isolated from the skeletal muscle of Sprague-Dawley neonatal rats　aged 3 days old.Cells at passage 5 were incubated in Dulbecco's modified Eagle's medium　supplemented with 10％ (v/v) fetal bovine serum,20 μg/L nerve growth factor,20 μg/L basic　fibroblast growth factor and 1％ (v/v) penicillin for 6 days.Cells presented with long processes,　similar to nerve cells.Connections were formed between cell processes.Immunocytochemical　staining with neuron specific enolase verified that cells differentiated into neuron-like cells.　Immunofluorescence cytochemistry and western blot results revealed that the expression of protein　hairy enhancer of split-1 was significantly reduced.These results indicate that low expression of　protein hairy enhancer of split-1 participates in the differentiation of muscle-derived stem cells into　neuron-like cells.