Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine

二维码有效期 120s

摘要Hyperhomocysteinemia is an important risk factor for preeclampsia-eclampsia.This study established a pregnant rat model of hyperhomocysteinemia,in which blood plasma homocysteine concentrations were twice or three times greater than that of normal pregnant rats.TUNEL revealed an increase in the number of apoptotic cells in the frontal cortex of pregnant rats with hyperhomocysteinemia.In addition,immunohistochemical staining detected activated nuclear factor-κB-positve cells in the frontal cortex.Reverse transcription-PCR detected that mRNA expression of the anti-apoptotic gene bcl-2diminished in the frontal cortex.In situ hybridization and western blotting revealed that N-methyl-D-aspartate receptor 1 mRNA and protein expression was upregulated in the frontal cortex and hippocampus.These results indicate that hyperhomocysteinemia can induce brain cell apoptosis,increase nerve excitability,and promote the occurrence of preeclampsia in pregnant rats.

作者 Jun Wang ^[1] Jing Ge ^[2] Liu Yang ^[3] Haiyan Zhang ^[2] Xuli Li ^[2] Dan Xue ^[2] 学术成果认领

作者单位 Department of Gynaecology and Obstetrics, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China ^[1] Department of Gynaecology and Obstetrics, the 202 Hospital of Chinese PLA, Shenyang 110003, Liaoning Province, China ^[2] Shenyang Women and Children Health Center, Shenyang 110014, Liaoning Province, China ^[3]

关键词 hyperhomocysteinemia homocysteine preeclampsia frontal cortex N-methyl-D-aspartate receptor nerve excitability cell apoptosis pregnancy brain neural regeneration

分类号 R714.244

栏目名称 Research and Reports

DOI 10.3969/j.issn.1673-5374.2012.028.005

发布时间 2012-12-20

基金项目

This project was funded by the General Project of Medical Technology of Military during the "12th Five-Year Plan" Period

翻译满意度评价：

准确一般字义偏差语意偏离译文作用小提交