Pannexin 1, a large-pore membrane channel,contributes to hypotonicity-induced ATP release in Schwann cells

二维码有效期 120s

摘要Pannexin 1 (Panx 1), as a large-pore membrane channel, is highly permeable to ATP and other signaling molecules. Previous studies have demonstrated the expression of Panx 1 in the nervous system, including astrocytes, microglia, and neurons. However, the distribution and function of Panx 1 in the peripheral nervous system are not clear. Blocking the function of Panx 1 pharmacologically (carbenoxolone and probenecid) or with small interfering RNA targeting pannexins can greatly reduce hypotonicity-induced ATP release. Treatment of Schwann cells with a Ras homolog family member (Rho) GTPase inhibitor and small interfering RNA targeting Rho or cytoskeleton disrupting agents, such as nocodazole or cytochalasin D, revealed that hypotonicity-induced ATP release depended on intracellular RhoA and the cytoskeleton. These findings suggest that Panx 1 participates in ATP release in Schwann cells by regulating RhoA and the cytoskeleton arrangement. This study was approved by the Animal Ethics Committee of Nantong University, China (No. S20180806-002) on August 5, 2018.

作者 Zhong-Ya Wei ^[1] Hui-Lin Qu ^[1] Yu-Juan Dai ^[2] Qian Wang ^[1] Zhuo-Min Ling ^[2] Wen-Feng Su ^[1] Ya-Yu Zhao ^[1] Wei-Xing Shen ^[2] Gang Chen ^[3] 学术成果认领

作者单位 Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China ^[1] Medical School of Nantong University, Nantong, Jiangsu Province, China ^[2] Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China;Medical School of Nantong University, Nantong, Jiangsu Province, China;Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China ^[3]

关键词 ATP cytoskeleton injury neuron pannexin 1 peripheral nerve Ras homolog family member A Schwann cells

分类号 R446 R741 Q493.8

栏目名称 Peripheral Nerve Injury and Neural Regeneration

发布时间 2020-11-17

基金项目

This study was supported by the National Natural Science Foundation of China,Nos.31900718 the National Key&nbsp Research and Development Program of China,No.2017YFA0104704(to GC) Basic Research Program of the Education Department of Jiangsu Province of China,Nos.19KJB180024 Postdoctoral Science Foundation of China, Innovation and Entrepreneurship Training Program of China,No.201810304031Z(to YJD) Six Talent Peaks Project in Jiangsu Province of China,No.WSN-007(to WXS)

翻译满意度评价：

准确一般字义偏差语意偏离译文作用小提交