摘要Studies have shown that gut microbiota metabolites can enter the central nervous system via the blood-spinal cord barrier and cause neuroinflammation,thus constituting secondary injury after spinal cord injury.To investigate the correlation between gut microbiota and metabolites and the possible mechanism underlying the effects of gut microbiota on secondary injury after spinal cord injury,in this study,we established mouse models of T8-T10 traumatic spinal cord injury.We used 16S rRNA gene amplicon sequencing and metabolomics to reveal the changes in gut microbiota and metabolites in fecal samples from the mouse model.Results showed a severe gut microbiota disturbance after spinal cord injury,which included marked increases in pro-inflammatory bacteria,such as Shigella,Bacteroides,Rikenella,Staphylococcus,and Mucispirillum and decreases in anti-inflammatory bacteria,such as Lactobacillus,Allobaculum,and Sutterella.Meanwhile,we identified 27 metabolites that decreased and 320 metabolites that increased in the injured spinal cord.Combined with pathway enrichment analysis,five markedly differential amino acids(L-leucine,L-methionine,L-phenylalanine,L-isoleucine and L-valine)were screened out,which play a pivotal role in activating oxidative stress and inflammatory responses following spinal cord injury.Integrated correlation analysis indicated that the alteration of gut microbiota was related to the differences in amino acids,which suggests that disturbances in gut microbiota might participate in the secondary injury through the accumulation of partial metabolites that activate oxidative stress and inflammatory responses.Findings from this study provide a new theoretical basis for improving the secondary injury after spinal cord injury through fecal microbial transplantation.