摘要Objective: To explore the influence of centrifugal parameters, cen-trifugal temperature, preparation equipment and anticoagulant on the manual prepa-ration of platelet-rich plasma(PRP). Methods: Female ICR mice (n=36) were divid-ed into 4 groups according to four influencing factors of centrifugation parameters (centrifugation force and centrifugation time), centrifugation temperature, preparation device, and anticoagulant. The red blood cell concentration (RBC), white blood cell concentration (WBC) and platelet concentration (PLT) were detected by blood cell analyzer, and the platelet enrichment factor (PEF) and platelet recovery ratio (PRR) were calculated. Results: In the comparison of the calculation results of PLT, PEF and PRR in each group, the centrifugal parameters were higher at 300 g for 10 min and 1200 g for 15 min, which were 2600±201.55, 3.68±1.29, 61.60％±8.57％(P<0.05);When the centrifugal temperature was 4℃, the tunica albuginea was flatter and easier to be absorbed than the normal temperature, and the RBCs between the groups were 0.84 ±0.33 and 3.16 ±0.33, respectively, with significant difference. There was no sig-nificant difference among the three data groups of anticoagulants, and the choice of anticoagulants did not have too much influence. The quality of PRP obtained from the improved preparation device (anticoagulant+syringe) was significantly higher than that of the anticoagulant tube, and the results were 2568 ±124.52 and 3551.67 ±348.12, 3.31 ±0.16 and 4.58 ±0.45, 49.7％±2.41％ and 68.74％±6.74％, respectively. Conclu-sion:High-quality platelet-rich plasma can be obtained by using an improved extrac-tion device with any anticoagulant at 4℃ and centrifugation parameters of 300 g for 10 min and 1200 g for 15 min.