摘要Objective:To investigate the effect of severe heatstroke on coagulation function in rats and the possible mechanism of high-mobility group protein B1(HMGB1)involved in the regulation of coagulation function.Methods:A total of 24 male SD rats were randomly divided into control group,mild group,moderate group and severe group,with 6 rats in each group.The rats in mild group,moderate group and severe group were continuously exposed to 40 ℃ and 10％humidity environment,then taken out and re-warmed to complete the heat stroke rat modeling process after the experiments were conducted for 70,110,and 145 min,respectively.The levels of HMGB1 in peripheral blood were detected by enzyme-linked immunosorbent assay(ELISA)before modeling and at 0 and 3 h after modeling,and coagulation tests were used to determine prothrombin time(PT),activated partial thromboplastin time(APTT),and prothrombin time(APT)at the same time points in the rats.The correlation between HMGB1 and coagulation parameters was determined by Pearson correlation.Results:In the severe group,HMGB1 was(2372.45±97.85)pg/ml and(2547.72±117.67)pg/ml at 0 and 3 h after modeling,respectively,and the levels of PT,APTT and Fib were higher than those before modeling and at 3 h after modeling.There was significant correlation between HMGBI and APTT,Fib(r=0.978,0.785,P=0.000.0.000).There were positive correla-tions between HMGBI and PT,APTT,Fib(r=0.634,0.976,0.889,P=0.001,0.000,0.000).Conclusion:HMGB1 may be involved in the pathogenesis of coagulation dysfunction in rats with severe heatstroke by regulating the activation of coagulation cells,and the level of HMGB1 in rats with severe heatstroke increases and still tends to increase after rewarming.