摘要Objective:To investigate the protective effect of cobra venom factor(CVF)on lung tissue injury in rats with sepsis induced by cecal puncture,with a view to providing theoretical basis for the development of new anti-sepsis drugs.Methods:A total of 144 rats were randomly divided into sham operation group,sepsis group and CVF group,with 48 rats in each group.Cecal ligation and puncture(CLP)was used to establish a rat model of sepsis.And 8 rats were taken from each group at 2,4,8,12,16 and 24 h to observe the differences in lung wet/dry weight ratio,lung histopathological changes,and serum inflammatory factor levels.Results:CVF treatment significantly alleviated the degree of pulmonary edema and swelling in septic rats,slowed down the trend of elevated lung wet/dry weight ratio,and reduced the pathological damage of lungs.The degree of vascular congestion,edema and inflammatory cell infiltration in lung tissue of the sepsis group increased with time,and the degree of lesions in the CVF group was milder than that in the sepsis group.The TNF-α level in lung tissue of septic rats was significantly down-regulated by CVF treatment,and the TNF-α levels in the CVF group during the 8-24 h period ranged from[8 h:(1.47±0.14)ng/ml]to[24 h:(1.14± 0.03)ng/ml],which was closer to that in sham operation group.Meanwhile,the 1L-10 level decreased from(91.3±5.5)ng/L to(39.8±13.4)ng/L during 8-24 h,which was higher than other groups.Conclusion:CVF can play a protective role against injury by down-regulating TNF-α and up-regulating IL-10 pathways,which can serve to alleviate focal lung injury and pulmonary edema from sepsis.