摘要Objective:To evaluate the performance parameters of adenosine triphos-phat(ATP)bioluminescence detection technology in assessing surface cleanliness in surgical environments and on hand surfaces.Methods:Samples were collected from 20 orthopedic surgeons at five hand sites(fingertips,hypothenar eminence,thenar eminence,nail grooves of the index/middle fingers,and nail matrices)before and after handwashing.ATP bioluminescence assays and agar plate colony counting were performed to measure relative light unit(RLU)values and logarithmic total colony counts,respectively.Differences in RLU and colony counts across hand sites were compared.Linear regression analysis was conducted to assess the correlation between RLU values and log-transformed colony counts at each site.The water Raman signal-to-noise ratio(S/N)and detection limits were also determined.Results:A significant linear positive correlation was observed between RLU values and log colony counts on surgeons'hands,with the regression equation:y=-0.4580+0.127x(p<0.001,R2=0.947).The sodium fluorescein standard demonstrated a water Raman S/N of 935.67 and detection limits of 0.86-51.53 RFU,both exceeding blank controls,with a wide linear range.Conclusion:ATP bioluminescence technology exhibits high sensitivity and precision for evaluating hand surface cleanliness,showing strong linear correlation with colony counts.Its detection limits surpass conventional standards,making it a robust tool for surgical hygiene monitoring.