摘要Objective:To construct a microneedle array engineered patch system loaded with insulin,and investigate its transdermal delivery efficiency and blood glu-cose regulation effect in diabetic model animals.Methods:PLGA microneedle arrays were prepared by micro-molding and loaded with insulin via coating method.The morphology,mechanical strength and in vitro release characteristics of the micronee-dles were characterized.Using streptozotocin-induced diabetic rats as models,blood glucose changes and pharmacokinetic parameters were compared between the mi-croneedle patch and subcutaneous injection.Results:The prepared microneedle ar-rays showed uniform morphology and sharp tips,which could penetrate the stratum corneum of rat skin.The cumulative insulin release rate in vitro reached 85.75%at 24 h.Animal experiments showed that the microneedle patch took effect at 30 min after ad-ministration,with a maximum blood glucose reduction of 58.3%,a duration of action up to 12 h,and a bioavailability of 82.5%compared with the subcutaneous injection group.Conclusion:The insulin-loaded microneedle array patch can achieve effective transdermal drug delivery and exhibit favorable blood glucose regulation in diabetic model rats,providing a novel approach for non-injectable insulin administration.