摘要目的 探讨苯扎氯铵(BAC)对无血清培养法培养的人结膜上皮细胞的细胞毒性.方法 采用无血清培养的3-5代人结膜上皮细胞,将不同浓度的BAC(O.01%,0.005%,0.001%,0.0005%,0.0001%)单次作用于结膜上皮细胞15min,分别于处理细胞后6,12,24,48hMTT法检测细胞活力,于处理细胞后48h固定,扫描电镜观察拍照.结果 自BAC处理后6h起,与对照组相比,各浓度BAC均使细胞活力下降(P＜0.01).经过48小时的恢复期,细胞活力未见恢复.随BAC浓度增高,细胞表面微绒毛消失,细胞出现不同程度收缩至脱落至大部分溶解坏死.结论 BAC对于培养的人结膜上皮细胞具有细胞毒性,其毒性作用呈剂量依赖性.

abstractsObjective To investigate the cytotoxic effects of benzalkonium chloride (BAC), a preservative used in many ophthalmic topical solutions, on human conjunctival epithelial cells in vitro.Methods Cultured epithelial cells obtained from human conjunctiva were exposed to medium containing BAC solutions at 0.01%, 0.005%, 0.001%, 0.0005% and 0.0001% concentrations for a period of 15 min. Cell were examined before treatment and 6,12, 24, 48 hours later, cell viability was assessed with methyl thiazolyl tetrazolium(MTT) assay, and scanning electron microscopy was used to evaluate the cytotoxic effect 48 hours after treatment.Results Cell viability decreased significantly in a dose-dependent manner after a single 15-minute treatment with BAC at various concentrations (P＜0.01 at all points after treatment). Cells treated with BAC at different concentrations showed loss of micromilli, cell exfoliation, even death. Conclusions These results suggest that BAC induces cytotoxicity at a concentration as low as 0.0001%. The mode of BAC-induced cytotoxicity is dose-dependent.