摘要Steroid saponins are secondary metabolites with multiple medicinal values that are found in large quantities in natural medicines,especially Vernonia amygdalina,a famous nature medicine for the treatment of tonsillitis,diabetes,pneumonia.The cur-rent study was designed to combine molecular networking(MN)with diagnostic ions for rapid identification of Δ7,9(11)stigmastane-type saponins which were the α-glucosidase inhibitory active substances in V.amygdalina.First,the α-glucosidase inhibitory activities of five Δ7,9(11)stigmastane-type steroid saponins that were previously isolated were screened,which indicated that the Δ7,9(11)stigmastane-type steroid saponin was one of the active constituents responsible for ameliorating diabetes.Furthermore,a strategy was proposed to identify stigmastane-type steroid saponins and verify the plausibility of derived fragmentation pathways by applying MN,MolNetEn-hancer and unsupervised substructure annotation(MS2LDA).Based on this strategy,other seven Δ7,9(11)stigmastane-type steroid saponins were identified from this plant.Our research provide scientific evidence for the antidiabetic potential of the steroid saponin-rich extract of V.amygdalina leaf.