摘要Nigella sativa L.seeds have been traditionally utilized in Chinese folk medicine for centuries to treat vitiligo.This study revealed that the ethanolic extract of Nigella sativa L.(HZC)en-hances melanogenesis and mitigates oxidative stress-induced cellular senescence and dys-function in melanocytes.In accordance with established protocols,the ethanol fraction from Nigella sativa L.seeds was extracted,concentrated,and lyophilized to evaluate its herbal ef-fects via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assays,tyros-inase activity evaluation,measurement of cellular melanin contents,scratch assays,senes-cence-associated β-galactosidase(SA-β-gal)staining,enzyme-linked immunosorbent assay(ELISA),and Western blot analysis for expression profiling of experimentally relevant pro-teins.The results indicated that HZC significantly enhanced tyrosinase activity and melanin content while notably increasing the protein expression levels of Tyr,Mitf,and gp100 in B16F10 cells.Furthermore,HZC effectively mitigated oxidative stress-induced cellular senes-cence,improved melanocyte condition,and rectified various functional impairments associ-ated with melanocyte dysfunction.These findings suggest that HZC increases melanin syn-thesis in melanocytes through the activation of the MAPK,PKA,and Wnt signaling pathways.In addition,HZC attenuates oxidative damage induced by H2O2 therapy by activating the nuc-lear factor E2-related factor 2-antioxidant response element(Nrf2-ARE)pathway and enhan-cing the activity of downstream antioxidant enzymes,thus preventing premature senescence and dysfunction in melanocytes.