摘要Eight previously undescribed lanostane triterpenoids,including five nortriterpenoids with 26 carbons,ganothenoids A-E(1-5),and three lanostanoids,ganothenoids F-H(6-8),along with 24 known ones(9-32),were isolated from the fruiting bodies of Ganodrma theaecolum.The structures of the novel compounds were elucidated using comprehensive spectroscopic methods,including electronic circular dichroism(ECD)and nuclear magnetic resonance(NMR)calculations.Compounds 1-32 were assessed for their neuroprotective effects against H2O2-induced damage in human neuroblastoma SH-SY5Y cells,as well as their inhibitory activities against protein tyrosine phosphatase 1B(PTP1B)and α-glucosidase.Compound 4 demonstrated the most potent neuroprotective activity against H2O2-induced oxidative stress by suppressing G0/G1 phase cell cycle arrest,reducing reactive oxygen species(ROS)levels,and inhibiting cell apoptosis through modulation of B-cell lymphoma 2 protein(Bcl-2)and Bcl-2 associated X-protein(Bax)protein expression.Compounds 26,12,and 28 exhibited PTP1B inhibitory activities with IC50 values ranging from 13.92 to 56.94 μmol·L-1,while compound 12 alone displayed significant inhibitory effects on α-glucosidase with an IC50 value of 43.56μmol·L-1.Additionally,enzyme kinetic analyses and molecular docking simulations were con-ducted for compounds 26 and 12 with PTP1B and α-glucosidase,respectively.