摘要Metastasis remains the primary cause of cancer-related mortality worldwide.Circulating tu-mor cells(CTCs)represent critical targets for metastasis prevention and treatment.Tradition-al Chinese medicine may prevent lung cancer metastasis through long-term intervention in CTC activity.Tiao-Shen-Zhi-Ai Formular(TSZAF)represents a Chinese medicine compound prescription utilized clinically for lung cancer treatment.This study combined three principal active ingredients from TSZAF into a novel TSZAF monomer combination(TSZAF mc)to in-vestigate its anti-metastatic effects and mechanisms.TSZAF me demonstrated significant in-hibition of proliferation,migration,and invasion in CTC-TJH-01 and LLC cells,while inducing cellular apoptosis in vitro.Moreover,TSZAF me substantially inhibited LLC cell growth and metastasis in vivo.Mechanistically,TAZSF me significantly suppressed the Wnt/β-catenin sig-naling pathway and CXCL5 expression in lung cancer cells and tissues.Additionally,TAZSF me notably reduced neutrophil infiltration in metastatic lesions.These findings indicate that TSZAF me inhibits lung cancer growth and metastasis by suppressing the Wnt/β-catenin sig-naling pathway and reducing CXCL5 secretion,thereby decreasing neutrophil recruitment and infiltration.TSZAF me demonstrates potential as an effective therapeutic agent for lung cancer metastasis.