摘要Ten previously undescribed flavonoids,seladoeflavones J-Q,C,and E(1-10),together with fifteen known biflavones(11-25),were isolated from the whole herbs of Selaginella doeder-leinii.The structures of the new compounds were elucidated using 1D and 2D nuclear magnet-ic resonance(NMR)spectroscopy and mass spectrometry(MS).By comparing experimental spectral data with NMR calculations,the structures of compounds 1-5,7,and 8 were as-signed.The absolute stereochemistries of compounds 5-10 were confirmed by comparing their circular dichroism(CD)spectra with reported data.Notably,the originally proposed structures of seladoeflavones C and E were revised and found to be identical to those of com-pounds 7 and 8,respectively.All isolated compounds were evaluated for cytotoxic activity against a panel of cancer cell lines.Most notably,2",3"-dihydroochnaflavone(14)and in-volvenflavone G(19)exhibited significant anti-proliferative and pro-apoptotic effects in laryngeal cancer cells(Hep-2 and FaDu).Mechanistic studies revealed that compound 14 in-duced apoptosis by suppressing the protein kinase B(Akt)/mammalian target of rapamycin(mTOR)signaling pathway,whereas compound 19 downregulated endoplasmic reticulum(ER)stress pathways.These findings indicate that compounds 14 and 19 possess strong po-tential as anti-laryngeal cancer agents,providing robust evidence for the traditional use of S.doederleinii in the treatment of laryngeal cancer.