摘要目的 探讨外源性碱性成纤维细胞生长因子(bFGF)对放射后大鼠学习记忆能力的影响.方法 利用分割照射建立的迟发性放射性脑损伤(RIB)模型鼠24只,采用尾静脉直接注射给药法给予外源性bFGF,3个月后进行学习记忆能力检测,同时观察脑组织病理变化及FGF受体1(FGFR1)表达情况.结果 bFGF组平均寻台潜伏期[(56.57±4.29)s]和第一象限路径与总路径长度百分比[(21.57±1.47)%]均较模型组[(118.24±10.79)s,(6.95±0.75)%]有显著改善(P<0.01);HE染色海马区域完整细胞计数bFGF组[(115.61±12.82)个]较模型组[(70.86±8.51)个]明显增多(P<0.01);IHC结果显示,bFGF治疗组海马内FGFR1表达水平(31.50±1.59)较模型组(12.03±1.68)明显增高(P<0.01).结论 外源性bFGF可通过诱导FGFR1表达对RIB模型鼠学习记忆能力起到一定的保护作用.

abstractsObjective To explore the therapeutic effects of basic fibroblast growth factor(bFGF) on learning and memory ability of post-radiation rats. Methods 24 Sprague-Dawley rats were treated with 30 Gy fractionated irradiation and then randomly divided into 2 groups. Exogenous hFGF and normal sodium were admin-istered intravenously through caudal vein after modeling. Morris maze tests were taken to study the learning and memory ability of rats 3 months after injection. The pathologic feature was studied by using H&E staining and IHC staining of FGF receptor 1 (FGFRI). Results Statistic differences of average escape latency and search strategy were found between model group [(118.24±10.79) s, (6.95±0.75)%] and bFGF group[(56.57±4.29) s, (21.57±1.47) %, P<0.01]. Intact cell number in hippocampus was more in bFGF group (115.61±12.82) than model group (70.86±8.51, P<0.01). There was weak expression of FGFR1 in hippocampus of model group(12.03±1.68) , which were fewer than bFGF group (31.50±1.59, P<0.01). Conclusion Exogenous bFGF injected intravenously through caudal vein has therapeutic effcets on learning and memory ability of post-radiation rats by inducing the expression of FGFR1.