摘要目的 观察不同剂量的布托啡诺硬膜外注射对大鼠神经功能及行为学的影响.方法 雄性SD大鼠,体质量180～210g,于L1-2处行硬膜外置入PE-530导管,3d后,取无运动障碍的大鼠32只,随机分为4组:生理盐水(NS)组(C组,n=8)、布托啡诺组(B1组n=8,B2组n=8,B3组n=8).C组硬膜外注射NS 30μl,B1、B2、B3组分别硬膜外注射布托啡诺60,120和240μg,都溶于30μl NS.置管成功后第1～5天给药,1次/d.在未给药时(T0)、第1～4天给药后6h(T1～4)及第5天给药后6h、24h、72h(T5～T7),各组行斜板试验和BBB(BASSO,BEATTTE,BRESNAHAN)评分.结果 布托啡诺60μg组在各时间点BBB评分和斜板试验和C组比较,差异无显著性(P＞0.05);B2组、B3组在T0～T3时间点,BBB评分和斜板试验和C组比较,差异无显著性(P＞0.05).随着时间的变化,给药后第4天(T4)BBB评分[(18.50±2.00)分、(16.38±2.33)分]和斜板试验[(58.75±5.17)°、(59.38±3.20)°]比C组[(21.00±0.00)分、(65.00±3.78)度]明显降低,差异有显著性(P＜0.05),并持续到T7.结论 布托啡诺60μg连续给药对大鼠神经功能及行为学无影响,但布托啡诺120μg和布托啡诺240μg连续给药会损害大鼠神经功能,产生行为学的影响.

abstractsObjective To observe the effect of epidural injection with different dose of butorphanol on the rats' neurological function.Methods A PE-530 catheter was inserted into the epidural space of all the SpragueDawley rats (male, weighting 180 ～210 g) at L1-2 level.After three days, a total of 32 rats without any motor dysfunction were randomly divided into 4 groups as follows saline(NS) group (group C, n= 8 )and butorphanol injection (B) group( B1∶ n=8;B2∶ n=8;B3∶ n=8).Rats in group C were epidurally injected NS 30 μl each ,and rats in group B1, B2 and B3 were respectively epidurally injected Butorphanol 60 μg/30μl, 120 μg/30 μl,240 μg/30 μl (all diluted with NS) ,and 1 time per day for5 days.The neurological function of rats was recorded before injection (T0) and 6h after injection on day 1 ～4(T1 ～T 4) and 6h,24h and 72h after injection on day 5 (T5 ～T7) by BBB (BASSO,BEATTIE and BRESNAHAN ) Score and the inclined plane test .Results Compared with group C ,the BBB score and the inclined plane test of group B1 showed no significant difference throughout the experimental period(P＞ 0.05 ).There was also no significant difference at T0 ～ T3 of group B2 and group B3 compared with group C (P ＞ 0.05 ), while at T4, the BBB score ( ( 18.50 ± 2.00 ) points, ( 16.38 ± 2.33 ) points) and the inclined plane test( (58.75 ± 5.17 )°, (59.38 ± 3.20) ° ) of the two groups were both obviously decreased when compared with group C( (21.00 ±0.00) points, (65.00 ±3.78)°, P＜0.05) ,and the same significant differences appeared at T5,T6 and T7 (P ＜ 0.05 ).Conclusion Repeated epidural injection of butorphanol 60 μg have no effect on neurological function of rats,while repeated epidural injection of butorphanol 120 μg and 240 μg could impaire the neurological function.