摘要目的 研究携带脑源性神经营养因子(BDNF)基因的人脐带间充质干细胞(UCMSC)脑内移植对脑损伤大鼠神经行为学恢复的影响.方法 建立大鼠自由落体颅脑损伤动物模型;培养纯化UCMSC,构建BDNF-pcDNA3重组质粒载体,并转染UCMSC;体外检测UCMSC携带BDNF基因的表达水平,并进行生物活性分析.将成功表达BDNF的UCMSC移植入大鼠损伤局部脑组织.2周进行神经功能缺损评分,探讨BDNF转基因UCMSC移植对脑损伤大鼠神经行为学的影响.结果 大鼠脑损伤后2周,神经功能[(10.50±0.53)分]障碍明显.UCMSC在损伤脑区存活,迁移;BDNF转基因UCMSC移植后神经功能[(5.50±0.76)分]与单纯UCMSC移植组[(7.75±0.71)分]比较明显改善,差异有统计学意义(P＜0.01).结论 携带BDNF基因的UCMSC脑内移植对脑损伤大鼠神经行为学恢复有明显促进作用.

abstractsObjective To explore the effect of brain derived neurtrophic factor(BDNF) gene modified umbilical cord mesenchymal stem cells ( UCMSC ) transplantation on neurological functional improvement in rats after brain trauma.Methods Cerebral contusion model in motor-sensory cortex in rats was established by a weight hammer falling method.UCMSC were cultured and transferred with BDNF gene.After BDNF expression and activity were determined,the BDNF gene modified UCMSC were implanted into traumatic brain.The neurological function was evaluated for 2 weeks after brain injury.And the BDNF expression was determined by using immunohistochemistry.Results Severe neurological dysfunction was seen in animals that had been subjected to contusion brain injury( 10.50 ±0.53 ).A significant improvement on neurological function was found in the UCMSC transplantaion animal( 7.75 ± 0.71 ), compared with only brain injury group (P ＜ 0.01 ).Moreover, rats in BDNF gene modified UCMSC showed the most behavior improvement ( 5.50 ± 0.76 ) (P ＜ 0.01 ).Conclusion BDNF gene modified UCMSC transplantation can survive and migrate, and improve neurological function in brain traumatic rats.