摘要目的 研究丁苯酞(NBP)对洒精依赖大鼠海马硫化氢(H2S)含量及N-甲基-D-天冬氨酸(NMDA)受体2B亚基(NR2B)表达的影响.方法 84只SD雄性大鼠随机分为6组,除正常组外,各组饮含6%(V/V)酒精水溶液28 d,饮6%(V/V)酒精水溶液14 d后,NBP低、中、高剂量组大鼠灌胃不同剂量NBP,检测戒断症状评分、海马组织H2S含量、胱硫醚β-合酶(CBS)活性以及NR2B的表达.结果 NBP中剂量组大鼠戒断症状评分[(12.27±1.19)分]、H2S含量[(30.25±8.82)nmol/g]、CBS活性[(72.44 ±7.46)nmol·g-1·h-1]及NR2B mRNA的表达(19.47 ±0.86)与实验对照组大鼠戒断症状评分[(14.09±2.21)分]、H2S含量[(44.50±6.65)nmol/g]、CBS活性[(79.06±4.57)nmol·g-1·h-1]及NR2B mRNA的表达(29.13±1.39)相比均降低,差异有显著性(P<0.05).NBP高剂量组大鼠戒断症状评分[(12.18±1.08)分]、H2S含量[(33.00±5.38)nmol/g]、CBS活性[(67.81±9.37)nmol·g-1·h-1]及NR2B mRNA的表达(23.12 ±1.86)与实验对照组大鼠相比均降低,差异有显著性(P<0.05).结论 NBP能够减轻酒精依赖大鼠的戒断症状,可能与NBP抑制H2S/CBS体系表达及抑制NR2B mRNA表达有关.

abstractsObjective To investigate the effect of butylphthalide (NBP) on H2S content and the expression of NR2B in the hippocampus of alcohol dependence rats. Methods A total of 84 SD male rats were randomly divided into 6 groups. Except for the normal group, other groups were subjected to alcohol solution with concentration of 6% ( V/V) for 28 d. Drug intervention began at the 14th day,and rats in the low,medium,high dose group were treated with NBP with a different concentration. Erden abstinence scoring was used to evaluate the rats withdrawal symptom. H2S content was measured in one side of hippocampus and CBS activity was tested in the other side of hippocampus. Hippocampus of 3 rats from each group was used to investigate NR2B mRNA level. Results Withdrawal symptom score ( 12.27 ± 1. 19),H2S content(30. 25 ±8.82), CBS activity (72. 44 ±7. 46) and NR2B mRNA expression( 19. 47 ±0. 86) in medium dose NBP group rats were lower than withdrawal symptom score(14.09 ±2.21) ,H2S content(44. 50 ±6. 65) , CBS activity(79. 06 ±4. 57) and NR2B mRNA expression (29. 13 ±1.39) in experimental control group (P<0.05). Withdrawal symptom score(12. 18 ±1.08) ,H2S content(33.00 ±5.38) ,CBS activity(67. 81 ±9. 37) and NR2B mRNA expression(23. 12 ± 1. 86) in high dose NBP group rats were lower than experimental control group (P < 0. 05). Conclusion NBP can reduce withdrawal symptoms of alcohol dependence rats,may be related to decreased expression of H2S/CBS system, and NR2B mRNA expression.