摘要目的 探究不同剂量的催产素对于Wistar孕鼠应激模型攻击行为的影响.方法 将40只孕鼠按随机数字表分为实验组(A、B、C)与对照组(D),每组10只;其中,A、B、C三组每次强迫游泳应激前分别经腹腔给予0.3 mg/kg、1.0 mg/kg、2.0 mg/kg的催产素,D组给予等体积生理盐水.各组待分娩第3天均进行母性攻击行为测试,主要观察母鼠的攻击总数、撕咬次数、攀压次数、攻击潜伏期、攻击持续时间等项目,比较各组攻击行为的差异,并用高架十字迷宫对各组大鼠在开臂、闭臂的相关数据进行分析比较.结果 A组与D组在攻击持续时间和潜伏期方面有差异统计学意义[(147.60±23.92)s、(79.70±9.88)s,P＜0.05],其他项目无差异统计学意义(P＞0.05);B组与D组除攻击总数(P＞0.05)外其他均有差异统计学意义[(3.10±0.87)次、(13.60±5.14)次、(91.30±9.74)s、(167.20±30.02)s,P＜0.01];C组与D组各指标均有差异统计学意义[(6.40±1.34)次、(15.10±4.35)次、(23.70±3.46)次、(49.80±5.53)s、(215.60±39.55)s,P＜0.01];A组与B组各指标有差异统计学意义(P＜0.05);B组与C组除攀压次数(P＞0.05),其他均有差异统计学意义(P＜0.01).在高架十字迷宫中,A组进入开臂次数百分率和进入开臂时间百分率较D组均差异有统计学意义(百分率数值分别为0.47±0.13、0.13±0.05,P＜0.01),B组与D组差异统计学意义(P＜0.01),A组与B组差异有统计学意义(P＜0.05),B组与C组在进入开臂时间百分率中无差异统计学意义(P＞0.05).结论 催产素可正性调节应激对母性攻击行为的影响,改善母仔亲密性,且催产素在此过程中具有剂量依从性.

abstractsObjective To explore the effect of the different doses of oxytocin(OXT) on stress model aggressive behaviour in Wistar rats.Methods According to random number table,40 pregnant rats were divided into experimental stress groups (A, B, C) and control group(D) with 10 samples in each group.Before forced swimming test, rats in experimental groups (A, B, C)were given OXT via intraperitoneal injection with 0.3 mg/kg, 1.0 mg/kg,2.0 mg/kg;rats in group D were given equal volume of normal saline in the same way.At the 3rd day all rats were tested with maternal aggression,including mothers' following indices as total aggression times,biting times,pinning times,attack latency and duration of attack.Elevated plus maze (EPM) test was applied to analyze the behavioral changes.Results In terms of the duration of attack and attack latency,the indexes of group A and group D were significantly different from relevant data ((147.60±23.92) s and(79.70±9.88) s, P＜0.05), while other indexes had no difference(P＞0.05).The duration of attack and attack latency had a significant difference ((3.10±0.87) times, (13.60±5.14)times, (91.30±9.74)s and(167.20±30.02) s, P＜0.01) between group B and group D except total aggression times(P＞0.05).Group C and group D in given indexes had significant differences showing (6.40± 1.34) times, (15.10±4.35) times, (23.70±3.46) times, (49.80± 5.53) s and (215.60± 39.55) s (P＜0.01).Group A and group B in given indexes had significant differences (P＜ 0.05);and group B and group C had a significant difference (P＜0.01)except pinning times(P＞0.05).In EPM ,the open arm entry ratio and the open arm time ratio of both group A and group D were significantly different from respective percentage (0.47±0.13) and (0.13±0.05) (P＜0.01).The significant difference was found in group B and group D(P＜0.01);group A and group B had a statistical difference(P＜0.05);there was no difference in both groups in open arm time ratio(P＞0.05).Conclusion Oxytocin potentially mediates maternal aggression by attenuating stress, and improves the intimate bonding between mother and pups,which means dose dependency of OXT in this process.