Crystallization scale purification of α7 nicotinic acetylcholine receptor from mammalian cells using a BacMam expression system

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作者单位 VARI-SIMM Center, Center for Structure and Function of Drug Targets, CAS-Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China ^[1] National Center for Protein Science Shanghai, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201210, China ^[2] VARI-SIMM Center, Center for Structure and Function of Drug Targets, CAS-Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China ^[3] Laboratory of Structural Sciences, Van Andel Research Institute, Grand Rapids, MI 49503, USA ^[4]

关键词 α7-nAChR membrane protein receptor expression receptor purification BacMam HEK293F cells

栏目名称

Drug Discovery

DOI 10.1038/aps.2015.34

发布时间 2015-08-25