摘要Higher drug loading employed in nanoscale delivery platforms is a goal that researchers have long sought after.But such viewpoint remains controversial because the impacts that nanocarriers bring about on bodies have been seriously overlooked.In the present study we investigated the effects of drug loading on the in vivo performance of PEGylated liposomal doxorubicin(PLD).We prepared PLDs with two different drug loading rates:high drug loading rate,H-Dox,12.9％w/w Dox/HSPC;low drug loading rate,L-Dox,2.4％w/w Dox/HSPC(L-Dox had about 5 folds drug carriers of H-Dox at the same Dox dose).The pharmaceutical properties and biological effects of H-Dox and L-Dox were compared in mice,rats or 4T1 subcutaneous tumor-bearing mice.We showed that the lowering of doxorubicin loading did not cause substantial shifts to the pharmaceutical properties of PLDs such as in vitro and in vivo stability(stable),anti-tumor effect(equivalent effective),as well as tissue and cellular distribution.Moreover,it was even more beneficial for mitigating the undesired biological effects caused by PLDs,through prolonging blood circulation and alleviating cutaneous accumulation in the presence of pre-existing anti-PEG Abs due to less opsonins(e.g.IgM and C3)deposition on per particle.Our results warn that the effects of drug loading would be much more convoluted than expected due to the complex intermediation between nanocarriers and bodies,urging independent investigation for each individual delivery platform to facilitate clinical translation and application.