摘要Immunosuppression by the tumor microenvironment is a pivotal factor contributing to tumor progression and immunotherapy resistance.Priming the tumor immune microenvironment(TIME)has emerged as a promising strategy for improving the efficacy of cancer immunotherapy.In this study we investigated the effects of noninvasive radiofrequency radiation(RFR)exposure on tumor progression and TIME phenotype,as well as the antitumor potential of PD-1 blockage in a model of pulmonary metastatic melanoma(PMM).Mouse model of PMM was established by tail vein injection of B16F10 cells.From day 3 after injection,the mice were exposed to RFR at an average specific absorption rate of 9.7 W/kg for 1 h per day for 14 days.After RFR exposure,lung tissues were harvested and RNAs were extracted for transcriptome sequencing;PMM-infiltrating immune cells were isolated for single-cell RNA-seq analysis.We showed that RFR exposure significantly impeded PMM progression accompanied by remodeled TIME of PMM via altering the proportion and transcription profile of tumor-infiltrating immune cells.RFR exposure increased the activation and cytotoxicity signatures of tumor-infiltrating CD8+T cells,particularly in the early activation subset with upregulated genes associated with T cell cytotoxicity.The PD-1 checkpoint pathway was upregulated by RFR exposure in CD8+T cells.RFR exposure also augmented NK cell subsets with increased cytotoxic characteristics in PMM.RFR exposure enhanced the effector function of tumor-infiltrating CD8+T cells and NK cells,evidenced by increased expression of cytotoxic molecules.RFR-induced inhibition of PMM growth was mediated by RFR-activated CD8+T cells and NK cells.We conclude that noninvasive RFR exposure induces antitumor remodeling of the TIME,leading to inhibition of tumor progression,which provides a promising novel strategy for TIME priming and potential combination with cancer immunotherapy.